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6 Well glass bottom plates

Author: Geym

Mar. 07, 2024

Packaging & Printing

  • A proposed mechanism for spontaneous transitions between interictal and ictal activity
    T Jacob, et al., Journal of Neuroscience 16 November 2018
    Quote: "Slices 285 were then transferred to membrane inserts (PICMORG50; Millipore), which were placed in 286 glass-bottomed six-well plates (P06-1.5HN, CellVis)."

  • The Autism Protein Ube3A/E6AP Remodels Neuronal Dendritic Arborization via Caspase-Dependent Microtubule Destabilization
    Natasha Khatri, et al., Journal of Neuroscience Vol. 38, Issue 2 10 Jan 2018
    Quote: "Briefly, dissociated neurons from rat hippocampus or cortex were seeded onto poly-l-lysine-coated (Sigma-Aldrich) coverslips in 60 mm dishes, each containing five coverslips, six-well plates, or glass-bottom six-well plates (In Vitro Scientific)"

  • 3D Bioprinting–Flow Cytometry as Analytical Strategy for 3D Cell Structures
    S Gretzinger, et al., Bioprinting Volume 11, September 2018
    Quote: "Round scaffolds of 9 mm in diameter and 4 horizontal and vertical lines (line spacing 2.25 mm) were printed up to a height of 3 mm directly into 6 well glass bottom plates (Cellvis, Mountain View, USA)"

  • Pooled optical screens in human cells
    D Feldman, et al., BioRxiv, August 02, 2018
    Quote: "Cells were then split for screening either via in situ sequencing or by FACS. For in situ screening, 500,000 cells were seeded into each well of a glass-bottom 6-well plate (CellVis)."

  • DISC1 regulates N-Methyl-D-Aspartate receptor dynamics: Abnormalities induced by a Disc1 mutation modelling a translocation linked to major mental illness
    lise Malavasi, et al., BioRxiv June 17, 2018
    Quote: "The astrocyte-containing cell culture inserts were then transferred to Poly-D-Lysine coated 6 well high precision (1.5H) glass bottom plates (In Vitro Scientific)"

  • Micro-scaled topographies direct differentiation of human epidermal stem cells
    S Zijl,et al., Acta Biomaterialia Volume 84, 15 January 2019, Pages 133-145
    Quote: "TopoChips were mounted onto glass 6-well plates (Cellvis) with ProLong Gold anti-fade reagent (Thermo Fisher Scientific). Images (20x objective) were acquired on"

  • Phosphatidylinositol 4,5-bisphosphate (PIP2) modulates afterhyperpolarizations in oxytocin neurons of the supraoptic nucleus
    MK Kirchner, et al., The Journal of Physiology, May 2017
    Quote: "Recording pipettes (4–8 MΩ) were pulled from borosilicate glass with an outer diameter of 1.5 mm using a P-1000 flaming/brown horizontal micropipette puller (Sutter … P06-1.5HN, In Vitro Scientific, Sunnyvale, CA, USA)"

  • Nuclear Microenvironments Modulate Transcription From Low-Affinity Enhancers
    Justin Crocker, et al., BioRxiv
    Quote: "Expanded gels containing embryos were imaged in 6-well glass bottom plates (Cellvis, Mountain View, California, USA) using a Zeiss LSM 800 confocal microscope"

  • Near infrared spectroscopic assessment of developing engineered tissues: correlations with compositional and mechanical properties
    A Hanifi, et al., Analyst
    Quote: "Seeded scaffolds were transferred into 6-well glass-bottom cell culture dishes (In Vitro Scientific, Sunnyvale, CA) with media changes"

  • Ptdins (4, 5) P2 (PIP2) modulates afterhyperpolarizations in oxytocin neurons of the supraoptic nucleus
    MK Kirchner, et al., The Journey of Physiology
    Quote: "Recording pipettes (4-8 MΩ) were pulled from borosilicate glass with an outer diameter of 1.5 mm using a P-1000 flaming/brown horizontal micropipette puller (Sutter ... P06-1.5HN, In Vitro Scientific, Sunnyvale, CA)"

Descriptions of 6 Well Cell Culture Plate

A 6-well cell culture plate is a common tool used in biological and biomedical research for growing and studying cells in vitro. It consists of a plastic dish with six individual wells or compartments. Each well is typically designed to hold a specific volume of cell culture medium and allows researchers to isolate and grow different cell samples or experiments simultaneously.

The wells in a 6-well plate are usually arranged in a grid pattern, with two rows and three columns. The plate itself is made of a cell culture-grade polystyrene material that is sterile and suitable for maintaining cell viability.

Features

  • Structure: A 6-well cell culture plate typically consists of six individual wells, each with its own separate compartment. The wells are usually round or square in shape and are arranged in a 2×3 pattern.
  • Well Size: The wells in a 6-well plate are larger compared to higher-density plates like 24-well or 96-well plates, allowing more cells to be cultured in each well.
  • Well-to-well Isolation: Each well in a 6-well plate is isolated from neighboring wells, preventing cross-contamination between different cell cultures.
  • Sterility: 6-well plates are usually sterile and come pre-treated with tissue culture-grade coatings to support cell attachment and growth.

Applications of 6 Well Cell Culture Plate

  • Cell Culture: The primary application of a 6-well cell culture plate is to grow and maintain various types of cells in a controlled environment. It is commonly used for cell expansion, differentiation studies, and long-term culture experiments.
  • Drug Screening: 6-well plates are widely used in pharmaceutical research for screening the effects of drugs or compounds on cell viability, proliferation, or function. Each well can be treated with different concentrations or combinations of compounds.
  • Cell-based Assays: Many cell-based assays, such as proliferation assays, cytotoxicity assays, apoptosis assays, and migration assays, can be performed using a 6-well plate. Multiple experimental conditions or cell types can be tested simultaneously.
  • Gene Expression Studies: Researchers can transfect or transduce cells with genetic material, such as plasmids or viral vectors, in a 6-well plate to study gene expression, protein production, or specific genetic manipulations.
  • Cell Imaging: The flat and transparent surface of a 6-well plate makes it suitable for microscopy and live cell imaging. Cells can be cultured directly on the plate or transferred onto glass coverslips for visualization and analysis.
  • Co-culture Experiments: Co-culturing different cell types in a 6-well plate allows researchers to investigate cell-cell interactions, cellular signaling pathways, and multicellular responses.

In summary, the 6-well cell culture plate offers high-quality, cell-friendly material, uniform well distribution, convenient lid design, and compatibility with standard equipment, making it a reliable and versatile tool for cell culture experiments in research and laboratory settings.

All of the cell culture plates, like the 6-well tissue culture plate and 12-well cell culture plate, are high-quality USP Class VI polystyrene with high transparency as raw material and are used for scientific research,  suspension cells, adherent cells, cell culture, and more.

6 Well glass bottom plates

6 Cell Culture Well Plate, Plasma Treatment

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